|
KMID : 1123920020160010150
|
|
Korean Journal of Oriental Physiology and Pathology
2002 Volume.16 No. 1 p.150 ~ p.153
|
|
|
Effects of Rhizoma Gastrodiae on Cultured Mouse Spinal Motor Neurons Damaged by Hydrogen Peroxide
|
|
Kim Hyung-Soo
Lee Yong-Suk
Son Il-Hong
Lee Jae-Kyoo
Son Young-Woo
Lee Jung-Hun
Lee Kang-Chang
Ryu Myeung-Hwan
Song Ho-Jun
Sung Kang-Keyng
Park Seung-Taeck
Lee Kap-Sang
|
|
|
Abstract
|
|
|
|
To elucidate the toxic effect of oxygen free radicals on cultured mouse spinal motor neurons damaged by hydrogen peroxide(H₂O₂)-induced neurotoxicity, we examined the neurotoxicity induced by oxygen radicals by NR assay when cultured spinal motor neurons were grown in the medium containing various concentrations of H₂O₂ for 6 hours. In addition, neuroprotective effects of herb extracts such Rhizoma Gastrodiae(RG), on H₂O₂-induced neurotoxicity in cultured spinal motor neurons were evaluated after cultured spinal motor neurons were preincubated with various concentrations of herb extract, RG for 2 hours before 50uM H₂O₂ for 6 hours. H₂O₂ decreased remarkably cell viability in dose-and time-dependent manner in these cultures, and also herb extract, RG increased cell viability of spinal motor neurons damaged by H₂O₂ in these cultures. From the above results, it is suggested that H₂O₂ was toxic in cultured spinal motor neurons derived from mouse, and RG was effective in blocking the neurotoxicity induced by oxidative stress in these cultures.
|
|
|
KEYWORD
|
|
|
Oxidative stress, Spinal motor neuron, Cell viability
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|